random hexamer primers (Kaneka Corp)
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Kaneka Corp
random hexamer primers
Random Hexamer Primers, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+hexamer+primers/hexamer+primers+random/pm41754085-83-79-82
Average 86 stars, based on 1 article reviews
Random Hexamer Primers, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+hexamer+primers/hexamer+primers+random/pm41754085-83-79-82
Average 86 stars, based on 1 article reviews
random hexamer primers - by Bioz Stars,
2026-10
86/100 stars
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Cell Culture:Article Title: Polyphenols Limit Cerebral Endothelial Cell Dysfunction Under Inflammatory Conditions Related to Oral and Gut Microbiota Article Snippet: The absorbance was read at 560 nm (TECAN, Männedorf, Switzerland). .. The cells were cultured in a 6-well plate (3.5 × 10 5 cells/well) in DMEM for 24 h. After removing the medium, the cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenol-rich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Total RNA was extracted using TRIzol TM (Invitrogen, ThermoFisher Scientific, Dardilly, France) and 4 μg of RNA were subjected to reverse transcription (RT) by using Article Title: Polyphenols Limit Cerebral Endothelial Cell Dysfunction Under Inflammatory Conditions Related to Oral and Gut Microbiota. Article Snippet: Briefly, cells were plated on a 96-well plate (1.0 × 104 cells/well) for 24 h. Then, the medium was removed and cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenolrich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Five hours before the experimental period ended, 20 μL of a solution of 5 mg/mL MTT reagent (Sigma-Aldrich) prepared in PBS were added to each well. .. The cells were cultured in a 6-well plate (3.5 × 105 cells/well) in DMEM for 24 h. After removing the medium, the cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenol-rich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Total RNA was extracted using TRIzolTM (Invitrogen, ThermoFisher Scientific, Dardilly, France) and 4 μg of RNA were subjected to reverse transcription (RT) by using Plant Extract:Article Title: Polyphenols Limit Cerebral Endothelial Cell Dysfunction Under Inflammatory Conditions Related to Oral and Gut Microbiota Article Snippet: The absorbance was read at 560 nm (TECAN, Männedorf, Switzerland). .. The cells were cultured in a 6-well plate (3.5 × 10 5 cells/well) in DMEM for 24 h. After removing the medium, the cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenol-rich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Total RNA was extracted using TRIzol TM (Invitrogen, ThermoFisher Scientific, Dardilly, France) and 4 μg of RNA were subjected to reverse transcription (RT) by using Article Title: Polyphenols Limit Cerebral Endothelial Cell Dysfunction Under Inflammatory Conditions Related to Oral and Gut Microbiota. Article Snippet: Briefly, cells were plated on a 96-well plate (1.0 × 104 cells/well) for 24 h. Then, the medium was removed and cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenolrich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Five hours before the experimental period ended, 20 μL of a solution of 5 mg/mL MTT reagent (Sigma-Aldrich) prepared in PBS were added to each well. .. The cells were cultured in a 6-well plate (3.5 × 105 cells/well) in DMEM for 24 h. After removing the medium, the cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenol-rich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Total RNA was extracted using TRIzolTM (Invitrogen, ThermoFisher Scientific, Dardilly, France) and 4 μg of RNA were subjected to reverse transcription (RT) by using Reverse Transcription:Article Title: Polyphenols Limit Cerebral Endothelial Cell Dysfunction Under Inflammatory Conditions Related to Oral and Gut Microbiota Article Snippet: The absorbance was read at 560 nm (TECAN, Männedorf, Switzerland). .. The cells were cultured in a 6-well plate (3.5 × 10 5 cells/well) in DMEM for 24 h. After removing the medium, the cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenol-rich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Total RNA was extracted using TRIzol TM (Invitrogen, ThermoFisher Scientific, Dardilly, France) and 4 μg of RNA were subjected to reverse transcription (RT) by using Article Title: Protective Effects of Antioxidant Polyphenols against Hyperglycemia-Mediated Alterations in Cerebral Endothelial Cells and a Mouse Stroke Model. Article Snippet: Scope: Hyperglycemia alters cerebral endothelial cell and blood-brain barrier functions, aggravating cerebrovascular complications such as stroke during diabetes.. Redox and inflammatory changes play a causal role.. This study evaluates polyphenol protective effects in cerebral endothelial cells and a mouse stroke model during hyperglycemia. Article Title: Punica granatum L. mesocarp suppresses advanced glycation end products (AGEs)- and H 2 O 2 -induced oxidative stress and pro-inflammatory biomarkers Article Snippet: The protective potential of pomegranate (Punica granatum L.) mesocarp extract (PME) on a cell model mimicking diabetes-like oxidative stress exposed to advanced glycation end products (AGEs) and hydrogen peroxide (H2O2) was investigated.. The polyphenolic antioxidant rich PME afforded protection to erythrocytes from AAPH radical-induced lysis and significantly reduced (P < 0.001) ROS production in 3T3-L1 preadipocytes by preventing AGEs-modulated decrease in intrinsic antioxidant enzymatic activities and by reducing NOX1 expression.. PME significantly inhibited protein carbonyl and AOPP levels following their increase in the presence of AGEs and H2O2 (P < 0.05). Article Title: Exposure to an organometal compound stimulates adipokine and cytokine expression in white adipose tissue Article Snippet: Total RNA was isolated with TrizolTM reagent (Gibco BRL, Gaithersburg, MD). .. Two μg of total RNA was reverse-transcribed (RT) using Article Title: Polyphenols Limit Cerebral Endothelial Cell Dysfunction Under Inflammatory Conditions Related to Oral and Gut Microbiota. Article Snippet: Briefly, cells were plated on a 96-well plate (1.0 × 104 cells/well) for 24 h. Then, the medium was removed and cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenolrich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Five hours before the experimental period ended, 20 μL of a solution of 5 mg/mL MTT reagent (Sigma-Aldrich) prepared in PBS were added to each well. .. The cells were cultured in a 6-well plate (3.5 × 105 cells/well) in DMEM for 24 h. After removing the medium, the cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenol-rich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Total RNA was extracted using TRIzolTM (Invitrogen, ThermoFisher Scientific, Dardilly, France) and 4 μg of RNA were subjected to reverse transcription (RT) by using Article Title: Effects of Porphyromonas gingivalis Bacteria on Inflammation, Oxidative Stress and Lipid Metabolism in Models of Obese db / db Mice and 3T3-L1 Adipose Cells Article Snippet: Total RNA from 3T3-L1 adipocytes was isolated with TRIzol (Ambion, Thermo Fischer Scientific, Waltham, MA, USA). .. Then, 1 μg of total RNA was reverse-transcribed using Random Hexamer:Article Title: Polyphenols Limit Cerebral Endothelial Cell Dysfunction Under Inflammatory Conditions Related to Oral and Gut Microbiota Article Snippet: The absorbance was read at 560 nm (TECAN, Männedorf, Switzerland). .. The cells were cultured in a 6-well plate (3.5 × 10 5 cells/well) in DMEM for 24 h. After removing the medium, the cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenol-rich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Total RNA was extracted using TRIzol TM (Invitrogen, ThermoFisher Scientific, Dardilly, France) and 4 μg of RNA were subjected to reverse transcription (RT) by using Article Title: Protective Effects of Antioxidant Polyphenols against Hyperglycemia-Mediated Alterations in Cerebral Endothelial Cells and a Mouse Stroke Model. Article Snippet: Scope: Hyperglycemia alters cerebral endothelial cell and blood-brain barrier functions, aggravating cerebrovascular complications such as stroke during diabetes.. Redox and inflammatory changes play a causal role.. This study evaluates polyphenol protective effects in cerebral endothelial cells and a mouse stroke model during hyperglycemia. Article Title: Punica granatum L. mesocarp suppresses advanced glycation end products (AGEs)- and H 2 O 2 -induced oxidative stress and pro-inflammatory biomarkers Article Snippet: The protective potential of pomegranate (Punica granatum L.) mesocarp extract (PME) on a cell model mimicking diabetes-like oxidative stress exposed to advanced glycation end products (AGEs) and hydrogen peroxide (H2O2) was investigated.. The polyphenolic antioxidant rich PME afforded protection to erythrocytes from AAPH radical-induced lysis and significantly reduced (P < 0.001) ROS production in 3T3-L1 preadipocytes by preventing AGEs-modulated decrease in intrinsic antioxidant enzymatic activities and by reducing NOX1 expression.. PME significantly inhibited protein carbonyl and AOPP levels following their increase in the presence of AGEs and H2O2 (P < 0.05). Article Title: Exposure to an organometal compound stimulates adipokine and cytokine expression in white adipose tissue Article Snippet: Total RNA was isolated with TrizolTM reagent (Gibco BRL, Gaithersburg, MD). .. Two μg of total RNA was reverse-transcribed (RT) using Article Title: Polyphenols Limit Cerebral Endothelial Cell Dysfunction Under Inflammatory Conditions Related to Oral and Gut Microbiota. Article Snippet: Briefly, cells were plated on a 96-well plate (1.0 × 104 cells/well) for 24 h. Then, the medium was removed and cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenolrich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Five hours before the experimental period ended, 20 μL of a solution of 5 mg/mL MTT reagent (Sigma-Aldrich) prepared in PBS were added to each well. .. The cells were cultured in a 6-well plate (3.5 × 105 cells/well) in DMEM for 24 h. After removing the medium, the cells were exposed to LPSs from P. gingivalis or E. coli (10 μg/mL) in the presence or absence of the polyphenol-rich plant extract (10 μM GAE) or epicatechin (10 μM) for 24 h. Total RNA was extracted using TRIzolTM (Invitrogen, ThermoFisher Scientific, Dardilly, France) and 4 μg of RNA were subjected to reverse transcription (RT) by using Article Title: Effects of Porphyromonas gingivalis Bacteria on Inflammation, Oxidative Stress and Lipid Metabolism in Models of Obese db / db Mice and 3T3-L1 Adipose Cells Article Snippet: Total RNA from 3T3-L1 adipocytes was isolated with TRIzol (Ambion, Thermo Fischer Scientific, Waltham, MA, USA). .. Then, 1 μg of total RNA was reverse-transcribed using Gene Expression:Article Title: Protective Effects of Antioxidant Polyphenols against Hyperglycemia-Mediated Alterations in Cerebral Endothelial Cells and a Mouse Stroke Model. Article Snippet: Scope: Hyperglycemia alters cerebral endothelial cell and blood-brain barrier functions, aggravating cerebrovascular complications such as stroke during diabetes.. Redox and inflammatory changes play a causal role.. This study evaluates polyphenol protective effects in cerebral endothelial cells and a mouse stroke model during hyperglycemia. |